AlanFung:Protocol/Genome Analyzer

From ZhangLabWiki
Jump to navigation Jump to search

Preparing Reagents for the Genome Analyzer

  • Genome analyzer Reagent Compartment & Reagent Position

File:Reagent Position.jpg

  • Position of each reagent in the Genome Analyzer
NOTE:LABEL ALL REAGENT CONTAINERS WITH THE APPROPRIATE NUMBER
Position    Reagent                Volume              Container
1           Incorporation Mix      Varies w/ cycle #   50ml amber tube
2           DI H2O                                     250ml bottle
3           Scanning Mix           50ml                50ml tube
4           High Salt Buffer                           250ml bottle
5           Incorporation buffer   150ml               250ml bottle
6           Cleavage Mix           50ml                50ml tube
7           Cleavage Buffer        70ml                250ml bottle