Kun:LabNotes/ExonomeSeq/2009-10-16

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Revision as of 23:04, 16 October 2009 by KunZhang (talk | contribs) (New page: ==Test the 1-adaptor ligation protocol== *Billy sent me two tubes of sheared NA12878 samples captured by #6&7. *I did size selection on ~80ul of the sheared DNA from tube 1 using the Size...)
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Test the 1-adaptor ligation protocol

  • Billy sent me two tubes of sheared NA12878 samples captured by #6&7.
  • I did size selection on ~80ul of the sheared DNA from tube 1 using the SizeSelect gel, and extract ~100bp and ~120bp fragments.
  • I did End-repair on the extracted DNAs without further purification.
  • I also did End-repair on another 80ul sheared DNA from tube 2 (prior to size selection).