AlanFung:LabNotes/Sequencing/2009-11-23

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Quantification of New amplified Phi-X and all libraries

  • Dilute concentration by 10 fold for Har_2, Har_5
  • Dilute New amplified PhiX to 1nM (Mix 2ul amp. PhiX with 164.97 ddH2O)
  • Keep P3, P5, P7 and CVF_N_D as is
Samples
PhiX-Stock 8/9 P7
PhiX-Stock 8/9 P7
PhiX(1nM) Har_2
PhiX(1nM) Har_2
8/9 P3 Har_5
8/9 P3 Har_5
8/9 P5 CVF_N_D
8/9 P5 CVF_N_D
  • split every 50ul rxn into 2 wells
98C 30 sec -> 20 cycles of (98C 10sec->64C 20sec->72C 20sec)->72C 5 min
X8 X1.1
PhiX Control DNA (10nM) 1.000
Syb_FP5 (100uM) 0.200 1.6 1.76
Syb_RP7 (100uM) 0.200 1.6 1.76
2X Phusion 25.000 200 220
50X SYBR Green I 0.200 1.6 1.76
H20 24.000 192 211.2