AlanFung:Protocol/Construction of Solexa sequencing Library(USER)

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Revision as of 16:37, 24 November 2009 by >Alan6017518 (→‎USER Digestion)
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Sequencing Library Construction with Single Adaptor Protocol

PCR with dUTP and 6.3NH2 Primer

1 well
H2O 47.4
Amp F6.3NH2(100uM) 0.2
Amp R6.3NH2 (100uM) 0.2
dUTP (1mM) 0.8
50X SYBG I 0.4
2X Master Mix 50
Template 1
Total 100

USER Digestion

DNA 30
USER 3
Total 33