Revision as of 04:31, 9 January 2011 by >Alan6017518
Name in original paper
|
Nick-name
|
Sequence (5\'->3\')
|
Sequence Modification
|
Reference
|
A_adapter_t |
Long Y-adaptor (top) |
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATC*T |
3'-Phosphorothioate bond |
[1]
|
A_adapter_b |
Long Y-adaptor (bottom) |
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTCTTCTGCTTG |
None |
[1]
|
|
PE_t_adapter |
Regular Y-adaptor (top) |
ACACTCTTTCCCTACACGACGCTCTTCCGATC*T |
3'-Phosphorothioate bond |
[2]
|
PE_b_adapter |
Regular Y-adaptor (bottom) |
/5Phos/GATCGGAAGAGCGGTTCAGCAGGAATGCCGAG |
5'-phosphorylation |
[2]
|
|
PCR_F |
Sol. Amp. (Forward) |
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATC*T |
3'-Phosphorothioate bond |
[2]
|
PCR_R |
Sol. Amp. (Reverse) |
CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATC*T |
3'-Phosphorothioate bond |
[2]
|
|
Syb_FP5 |
Sol. Quant. (Forward) |
ATGATACGGCGACCACCGAG |
None |
[2]
|
Syb_RP7 |
Sol. Quant. (Reverse) |
CAAGCAGAAGACGGCATACGAG |
None |
[2]
|
|
PE_t_N2 |
Y-adaptor for MmeI ligation (top) |
ACACTCTTTCCCTACACGACGCTCTTCCGATCTN*N |
3'-Phosphorothioate bond |
|
PE_N2 |
Y-adaptor for MmeI ligation (top) |
ACACTCTTTCCCTACACGACGCTCTTCCGATC*NN |
3'-Phosphorothioate bond |
|
PE_b_A |
Y-adaptor for MmeI ligation (bottom) |
/5Phos/AGATCGGAAGAGCGGTTCAGCAGGAATGCCGAG |
5'-phosphorylation |
|
PE_t_Met |
bis-seq(Forward) |
A/iMe-dC/A/iMe-dC/T/iMe-dC/TTT/iMe-dC//iMe-dC//iMe-dC/TA/iMe-dC/ACGACG/iMe-dC/T/iMe-dC/TT/iMe-dC/CGAT/iMe-dC/*T |
3-phosphorothiate, 5'-Methyl |
|
PE_b_Met |
bis-seq (Reverse) |
/5Phos/GATCGGAAGAGCGGTT/iMe-dC/AG/iMe-dC/AGGAATG/iMe-dC/CGAG |
5'-phosphorylated, 5'-Methyl |
|
|
RC_PE_t_adapter |
RC_Regular Y-adaptor with Biotin (top) |
/5Biosg/TTTTTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT |
5'-Biotin
|
RC_PE_b_adapter |
RC_Regular Y-adaptor with Biotin (bottom) |
/5Biosg/TTTTTTTCTCGGCATTCCTGCTGAACCGCTCTTCCGATC |
5'-Biotin
|
Small RNA Sequencing Primer |
Solexa-TSS-Seq |
CGACAGGTTCAGAGTTCTACAGTCCGACGATC |
|
|
Library Free Multiplexing Primers
Name
|
Nick-name
|
Sequence (5\'->3\')
|
Sequence Modification
|
Reference
|
Left Probe Linker, No barcode (forward) |
AmpF6.3Sol |
AATGATACGGCGACCACCGACACTCTCAGATGTTATCGAGGTCCGAC |
None |
None
|
Right Probe Linker, Barcode 1: CGTGAT (reverse) |
AmpR6.3Ind1 |
CAAGCAGAAGACGGCATACGAGATCGTGATGCTAGGAACGATGAGCCTCCAAC |
None |
None
|
Right Probe Linker, Barcode 2: ACATCG (reverse) |
AmpR6.3Ind2 |
CAAGCAGAAGACGGCATACGAGATACATCGGCTAGGAACGATGAGCCTCCAAC |
None |
None
|
Right Probe Linker, Barcode 3: GCCTAA (reverse) |
AmpR6.3Ind3 |
CAAGCAGAAGACGGCATACGAGATGCCTAAGCTAGGAACGATGAGCCTCCAAC |
None |
None
|
Right Probe Linker, Barcode 4: TGGTCA (reverse) |
AmpR6.3Ind4 |
CAAGCAGAAGACGGCATACGAGATTGGTCAGCTAGGAACGATGAGCCTCCAAC |
None |
None
|
Right Probe Linker, Barcode 5: CACTGT (reverse) |
AmpR6.3Ind5 |
CAAGCAGAAGACGGCATACGAGATCACTGTGCTAGGAACGATGAGCCTCCAAC |
None |
None
|
Right Probe Linker, Barcode 6: ATTGGC (reverse) |
AmpR6.3Ind6 |
CAAGCAGAAGACGGCATACGAGATATTGGCGCTAGGAACGATGAGCCTCCAAC |
None |
None
|
Right Probe Linker, Barcode 7: GATCTG (reverse) |
AmpR6.3Ind7 |
CAAGCAGAAGACGGCATACGAGATGATCTGGCTAGGAACGATGAGCCTCCAAC |
None |
None
|
Right Probe Linker, Barcode 8: TCAAGT (reverse) |
AmpR6.3Ind8 |
CAAGCAGAAGACGGCATACGAGATTCAAGTGCTAGGAACGATGAGCCTCCAAC |
None |
None
|
Right Probe Linker, Barcode 9: CTGATC (reverse) |
AmpR6.3Ind9 |
CAAGCAGAAGACGGCATACGAGATCTGATCGCTAGGAACGATGAGCCTCCAAC |
None |
None
|
Right Probe Linker, Barcode 10: AAGCTA (reverse) |
AmpR6.3Ind10 |
CAAGCAGAAGACGGCATACGAGATAAGCTAGCTAGGAACGATGAGCCTCCAAC |
None |
None
|
Right Probe Linker, Barcode 11: GTAGCC (reverse) |
AmpR6.3Ind11 |
CAAGCAGAAGACGGCATACGAGATGTAGCCGCTAGGAACGATGAGCCTCCAAC |
None |
None
|
Right Probe Linker, Barcode 12: TACAAG (reverse) |
AmpR6.3Ind12 |
CAAGCAGAAGACGGCATACGAGATTACAAGGCTAGGAACGATGAGCCTCCAAC |
None |
None
|
Sequencing reading primer |
AmpR6.3IndSeq |
GTTGGAGGCTCATCGTTCCTAGC |
None |
None
|
Multiplex primers for the N2-adaptor
Sequence ID
|
Sequence
|
PCR_R.N2Ind1 |
CAAGCAGAAGACGGCATACGAGATCGTGATCTCGGCATTCCTGCTGAACCGCTCTT
|
PCR_R.N2Ind2 |
CAAGCAGAAGACGGCATACGAGATACATCGCTCGGCATTCCTGCTGAACCGCTCTT
|
PCR_R.N2Ind3 |
CAAGCAGAAGACGGCATACGAGATGCCTAACTCGGCATTCCTGCTGAACCGCTCTT
|
PCR_R.N2Ind4 |
CAAGCAGAAGACGGCATACGAGATTGGTCACTCGGCATTCCTGCTGAACCGCTCTT
|
PCR_R.N2Ind5 |
CAAGCAGAAGACGGCATACGAGATCACTGTCTCGGCATTCCTGCTGAACCGCTCTT
|
PCR_R.N2Ind6 |
CAAGCAGAAGACGGCATACGAGATATTGGCCTCGGCATTCCTGCTGAACCGCTCTT
|
PCR_R.N2Ind7 |
CAAGCAGAAGACGGCATACGAGATGATCTGCTCGGCATTCCTGCTGAACCGCTCTT
|
PCR_R.N2Ind8 |
CAAGCAGAAGACGGCATACGAGATTCAAGTCTCGGCATTCCTGCTGAACCGCTCTT
|
PCR_R.N2Ind9 |
CAAGCAGAAGACGGCATACGAGATCTGATCCTCGGCATTCCTGCTGAACCGCTCTT
|
PCR_R.N2Ind10 |
CAAGCAGAAGACGGCATACGAGATAAGCTACTCGGCATTCCTGCTGAACCGCTCTT
|
PCR_R.N2Ind11 |
CAAGCAGAAGACGGCATACGAGATGTAGCCCTCGGCATTCCTGCTGAACCGCTCTT
|
PCR_R.N2Ind12 |
CAAGCAGAAGACGGCATACGAGATTACAAGCTCGGCATTCCTGCTGAACCGCTCTT
|
PCR_Fs |
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTC
|
N2IndSeq |
AAGAGCGGTTCAGCAGGAATGCCGAG
|
N2RevSeq(WRONG design) |
CTCGGCATTCCTGCTGAACCGCTCTT
|
N2RevSeq2 |
CTCGGCATTCCTGCTGAACCGCTCTTCCGATCT
|
|
Nextera Read Primer
Nextera Read 1 Primer: 5'-GCCTCCCTCGCGCCATCAGAGATGTGTATAAGAGACAG-3'
Nextera Read 2 Primer: 5'-GCCTTGCCAGCCCGCTCAGAGATGTGTATAAGAGACAG-3'
Nextera Index Read Primer: 5'-CTGTCTCTTATACACATCTCTGAGCGGGCTGGCAAGGCAGACCG-3'