AlanFung:LabNotes/CTCF/2010-1-15

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Revision as of 16:26, 15 January 2010 by >Alan6017518 (New page: ==Quantify Bisulfite Converted Samples with Qubit ssDNA Kit== ==On 50ng, 10ng, 5ng, 1ng, 0.5ng, 0.1ng & 0.05ng P21-200BP== 7 samples Prepare 7 Qubit Assay tubes Prepare 7*200ul dilute...)
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Quantify Bisulfite Converted Samples with Qubit ssDNA Kit

On 50ng, 10ng, 5ng, 1ng, 0.5ng, 0.1ng & 0.05ng P21-200BP

7 samples
Prepare 7 Qubit Assay tubes

Prepare 7*200ul diluted dye=840ul
Mix 4.2ul dye with 835.8ul buffer
Mix 2ul samples with 98ul diluted dye
vortex spin down and wait for 2 mins

Result

PCR quantification of Bisulfite treated samples using 3 paris of CHR primers

On 50ng, 10ng, 5ng, 1ng, 0.5ng, 0.1ng & 0.05ng P21-200BP