AlanFung:LabNotes/CTCF/2010-4-8
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Denaturing Gel Quantification
- 0.5ul low mass ladder + 4.5ul ddh2o + 5ul 2x TBU Buffer
- 2ul sample + 3ul ddh2o + 5ul 2X TBU Buffer
File:ZhangLab 2 2010-04-08 12hr 46min.jpg
- By comparing the gel image from the TBE Gel Quantification on 3/24/10, it appears that the TBE gel matrix hinders the migration of the double stranded library and gives a false size of the DNA, while the TBU gel shows that that the band at 100BP (original DNA size) 150BP and 180BP.