Alice:LabNotes/2010-4-23

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Revision as of 17:01, 23 April 2010 by >Zsakura2 (Created page with '=PCR re-amplification of the sequencing libraries with PAIR-END primer= *samples to be re-amplified: CViB, DF, foreskin, ips17A, ips17B, ips11B, Hfib 11 …')
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PCR re-amplification of the sequencing libraries with PAIR-END primer

  • samples to be re-amplified: CViB, DF, foreskin, ips17A, ips17B, ips11B, Hfib 11
                                                                     x4 well each
DNA (0.353ng/ul)           2.5ul
Phusion HF PCR Master Mix (2x): 	50ul
PCR_R (100uM):                    0.2ul
PCR_F (100uM):                     0.2ul
SYBR Green 50x:                  0.4ul 
H2O:                         48ul                
Total:                     	100ul
98C 30sec -> (98C 10sec -> 55C 45sec -> 72C 30sec) x2 cycles -> (98C 10 sec -> 72C 30sec) x 3cycles -> 72C 5min -> hold at 4C