Alice:LabNotes/2010-4-23
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PCR re-amplification of the sequencing libraries with PAIR-END primer
- samples to be re-amplified: CViB, DF, foreskin, ips17A, ips17B, ips11B, Hfib 11
x4 well each DNA (0.353ng/ul) 2.5ul Phusion HF PCR Master Mix (2x): 50ul PCR_R (100uM): 0.2ul PCR_F (100uM): 0.2ul SYBR Green 50x: 0.4ul H2O: 48ul Total: 100ul 98C 30sec -> (98C 10sec -> 55C 45sec -> 72C 30sec) x2 cycles -> (98C 10 sec -> 72C 30sec) x 3cycles -> 72C 5min -> hold at 4C