Comparison of Staining Dyes
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Purpose
To establish the best dye to use for any subsequent experiments in order to maintain consistency in results.
Methods
3 dyes were tested: Dapi, SYBR Green, and YOYO. Each dye was diluted to 2X concentration.
Sample tubes 1 and 2 were combined, filtered through a 5um filter, and used as a standardized sample.
For each dye:
- Use a clean 0.2mL tube - Add 7uL sample + 3uL 2X dye - Incubate for 10 minutes in the dark - Add 10uL water - Vortex and spin down
Each sample was plated and imaged under a fluorescence microscope using 10X magnification.
Results
Image 1: 2X Dapi
File:SYBR Green 10X Comparison.jpg
Image 2: 2X SYBR Green
[[image: YOYO_10X_Comparison.jpg
Image 3: 2X YOYO