Kun:LabNotes/Haplotyping/2010-8-18

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Revision as of 16:37, 18 August 2010 by KunZhang (talk | contribs) (Created page with '==Human chromosome preparation== ===Make buffers=== *55mM KCl + 5.5mM HEPES @ pH8.0 *RNase: 3mg/ml *DDT: 120mM *100mM MgSO4 *2.5% Triton X-100 *Cell lysis buffer: 20mM EDTA, 10mM…')
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Human chromosome preparation

Make buffers

  • 55mM KCl + 5.5mM HEPES @ pH8.0
  • RNase: 3mg/ml
  • DDT: 120mM
  • 100mM MgSO4
  • 2.5% Triton X-100
  • Cell lysis buffer: 20mM EDTA, 10mM Tris.HCl (pH 8.0), 200mM NaCl, 0.2% Triton X-100, 100mg/ml Protease