Kun:LabNotes/Haplotyping/2010-8-18
Jump to navigation
Jump to search
Human chromosome preparation
Make buffers
- 55mM KCl + 5.5mM HEPES @ pH8.0
- RNase: 3mg/ml
- DDT: 120mM
- 100mM MgSO4
- 2.5% Triton X-100
- Cell lysis buffer: 20mM EDTA, 10mM Tris.HCl (pH 8.0), 200mM NaCl, 0.2% Triton X-100, 100mg/ml Protease