Kun:LabNotes/GenomeSeq/2010-9-4

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Revision as of 04:46, 4 September 2010 by KunZhang (talk | contribs) (Created page with '==Repeat the ligation PCR with barcoded and non-barcoded primers== *Template: B.Jap-3.3 *PCR: Adapter ligased DNA 1 μl 0 1 μl 0 PCR_F(10uM)…')
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Repeat the ligation PCR with barcoded and non-barcoded primers

  • Template: B.Jap-3.3
  • PCR:
  Adapter ligased DNA          1 μl  0                    1 μl   0
  PCR_F(10uM)                  2 μl  2                    2 μl   2
  PCR_R(10uM)                  2 μl  2   PCR_R_ind3(10uM) 2 μl   2
  50X SYBR Green I           0.8 μl .8                   .8 μl  .8
  H2O                         37 μl 38                   37 μl  38
  2X Phusion HF MM            50 μl 50                   50 μl  50
                              -----
                             100 μl
  98C 30sec -> (98C 10sec -> 68C 15sec -> 72C 15sec) x 18 cycles.