Kun:LabNotes/GenomeSeq/2010-9-4
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Repeat the ligation PCR with barcoded and non-barcoded primers
- Template: B.Jap-3.3
- PCR:
Adapter ligased DNA 1 μl 0 1 μl 0 PCR_F(10uM) 2 μl 2 2 μl 2 PCR_R(10uM) 2 μl 2 PCR_R_ind3(10uM) 2 μl 2 50X SYBR Green I 0.8 μl .8 .8 μl .8 H2O 37 μl 38 37 μl 38 2X Phusion HF MM 50 μl 50 50 μl 50 ----- 100 μl 98C 30sec -> (98C 10sec -> 68C 15sec -> 72C 15sec) x 18 cycles.