Kun Zhang LabNote CpG 2007 Oct wk2
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Friday 2007-10-12
Fri.Exp.Design: Design padlock probes to capture CpG islands
I'm starting from the primers used in Taylor et al. Cancer Research, 2007, 67:8511-8. To convert these sequencing into padlock probes, I need to do the followning:
- Remove the barcodes at the end of the primers;
- Convert the right primers to their reverse complementary sequences, add Gs to the end so that the total length of the forward and reverse sequences are the same for all targets;
- Add common linkers to the two capturing arms.
- Starting primer file.
- Perl script to convert primers to padlocks.
- The first set of padlock probes.