Sam:LabNotes/Microbiome-new/2010-9-24

From ZhangLabWiki
Revision as of 15:40, 25 September 2010 by >Sam Chiang
Jump to navigation Jump to search

Sanger sequencing test on E.coli gDNA MDA amplicons

Background

  • To optimize the Sanger sequencing protocl for MDA amplicon validation
  • This protocol will also include the S1 nucelase debranching, DNA Pol.I reparing procedures, and compare the difference between with and without these treatments.

Procedures