Rui:Haplotying
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Background
Original: File:Design.ppt
Modified: File:Tn-based-haplotyping V1.1.ppt
Protocol
Step 1: Cell lysis
Collect 10 cells in 20ul lysis buffer.
Cell lysis buffer (1*) 200mM NaCl 20mM EDTA 10mM Tris-HCl (pH 8) 0.2% TritonX-100 (0.1 AU/ml Qiagen Proteinase 7.5AU)
Step 2: Proteinase and aliquot
Add Proteinase right before aliquot, 1ul per tube, to 20 tubes. Store at -80C.
Step 3: Tagmentation
gDNA | 1ul |
HMW buffer | 1ul |
transposomes | 1ul |
Nf water | 2ul |
Total | 5ul |
Primers
File:Primers for Tn-based-haplotyping.xls
2011
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