Rui:Haplotying

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Revision as of 23:18, 16 March 2011 by >RuiLiu
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Background

Original: File:Design.ppt

Modified: File:Tn-based-haplotyping V1.1.ppt

Protocol

Step 1: Cell lysis

Collect 10 cells in 20ul lysis buffer.

Cell lysis buffer (1*)
200mM NaCl
20mM EDTA
10mM Tris-HCl (pH 8)
0.2% TritonX-100
(0.1 AU/ml Qiagen Proteinase 7.5AU)

Step 2: Proteinase and aliquot

Add Proteinase right before aliquot, 1ul per tube, to 20 tubes. Store at -80C.

Step 3: Tagmentation

gDNA 1ul
HMW buffer 1ul
transposomes 1ul
Nf water 2ul
Total 5ul


Primers

File:Primers for Tn-based-haplotyping.xls

2011

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