Rui:LabNotes/SingleCell/2011-7-1

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Purification of HRCA results

MinElute

NC, PC, 60pg gDNA, 1pg lysate 1, elute in 10ul, 1ul for TBE PAGE

File:7.1.11 MinElute.jpg

Qbuit: ~1ng/ul

EtOH precipitation (ExoI treatment)

PC, 1pg lysate 2 (treated with ExoI for 30min)

PC, 1pg lysate 3 (no treatment)

EtOH precipitation: 5ul NaOAc, 125ul EtOH, -80C 30min, spin 30min, huge pellet, resuspend in 30ul

Qbuit: L2: 110ng/ul; L3: 10ng/ul; PC out of range (too high)

File:7.6.11 quant.jpg

File:7.6.11 over.jpg