Brandon Sos

From ZhangLabWiki
Revision as of 23:54, 26 February 2013 by >Bsos (→‎Main Calendar)
Jump to navigation Jump to search

Current Projects

Protocols

polyacrylamide DNA gel

TBU gel

Count cells

Primers prep

Lysis buffers

gel size selection

AMPure XP beads purification only (for >= 5 ng)

AMPure XP beads purification 2-step PCR (for <=5 ng)

Cloning and Transformation.

Invitrogen MAXIscript T7 In vitro transcription kit

SMART MMLV RT protocol (for first strand synthesis)

standard PCR cycling for for different enzymes etc

enzyme reaction buffer compositions

e gel protocol

qubit protocol


  • notes
    • minielute colums for <10 ul samples, otherwise use qiaquick columns
    • N2 adaptor is illuminia adaptor. Nextera adaptor is nextera adaptor


Scratch for notes etc

Main Calendar

<calendar> name=Brandon_Sos:LabNotes/Project1 format=%name/%year-%month-%day date=2013/02/01 view=twoyear </calendar>

<calendar> name=Brandon:LabNotes/Project1 format=%name/%year-%month-%day date=2012/02/01 view=twoyear </calendar>

<calendar> name=Brandon:LabNotes/Project1 format=%name/%year-%month-%day date=2011/02/01 view=twoyear </calendar>