Talk:Alice Li
Exp1: PCR on PGP1 and Hues6 gDNA/cDNA for Sanger sequencing
Summary of reactions: Reaction SNP PCR primer Primer location Sample 1(1A) rs3753565 rs3753565_F/rs3753565_R SnpVer2 A1/A12 Hues6 gDNA 2(1B) rs3753565 rs3753565_F/rs3753565_R SnpVer2 A1/A12 Hues6 cDNA 3(1C) rs2295680 rs2295680_F/rs2295680_R SnpVer2 A2/B1 PGP1 gDNA 4(1D) rs2295680 rs2295680_F/rs2295680_R SnpVer2 A2/B1 GM20431 cDNA 5(1E) rs2295680 rs2295680_F/rs2295680_R SnpVer2 A2/B1 GC1F cDNA 6(1F) rs2295680 rs2295680_F/rs2295680_R SnpVer2 A2/B1 GC1EP cDNA 7(1G) rs6103 rs6103_F/rs6103_R SnpVer2 A9/B8 PGP1 gDNA 8(1H) rs6103 rs6103_F/rs6103_R SnpVer2 A9/B8 GM20431 cDNA 9(2A) rs6103 rs6103_F/rs6103_R SnpVer2 A9/B8 GC1F cDNA 10(2B) rs6103 rs6103_F/rs6103_R SnpVer2 A9/B8 GC1EP cDNA 11(2C) rs6103 rs6103_F/rs6103_R SnpVer2 A9/B8 Hues6 gDNA 12(2D) rs6103 rs6103_F/rs6103_R SnpVer2 A9/B8 Hues6 cDNA 13(2E) rs1264899 rs1264899_cF/rs1264899_cR Hues6Ver2c F1/F4 PGP1 gDNA 14(2F) rs1264899 rs1264899_cF/rs1264899_cR Hues6Ver2c F1/F4 GM20431 cDNA 15(2G) rs1264899 rs1264899_cF/rs1264899_cR Hues6Ver2c F1/F4 GC1F cDNA 16(2H) rs1264899 rs1264899_cF/rs1264899_cR Hues6Ver2c F1/F4 GC1EP cDNA
Template: 10ng genomic DNA or 2ng cDNA, use new aliquots of templates/primers/SYBG I diluted in nuclease free H2O. ' one reaction total Template 1 17 10x Buffer 5 85 25mM MgCl2 3 51 10mM dNTP 1 17 10uM forward primer 1 17 10uM reverse primer 1 17 50x SYBG I 0.4 6.8 thermo start Tag 0.25 4.25 H2O 37.35 634.95
95C 3min -> 35 cycles of (95C 15S -> 58C 30S -> 72C 1min) -> 72C 3min