Noi/NOTES/2012-8-1

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WGBS sequencing library preparation using KAPA protocol (Eppendorf) and epMotion (Repeat!)

  • The step of library sequencing prep will be filled soon.

File:ZhangLab 2 2012-08-02 11hr 31min-HAPMAP-bis-test.jpg

  • Purified sequencing libraries with 0.8x vol AMPure bead with epMotion (3 strips), eluted with 25ul EB for each well -> total volume at the end ~70ul

File:ZhangLab 2 2012-08-02 16hr 05min bead-HAPMAP-WGBS.jpg

PAGE quantification of 400-600bp fragments

  • Perform PAGE size-selection of the size range 400-600bp for a small scale sequencing

File:ZhangLab 2 2012-08-02 16hr 05min bead-HAPMAP-WGBS-400-600bp PQ.jpg

Lab IDs Final conc.
of 400-600bp (ng/ul)
Total yields in 70ul (ng)
#1 13.50 945.28
#2 9.66 675.94
#3 11.24 786.94
#4 13.64 955.09
#5 13.85 969.55
#6 8.95 626.85
#7 12.40 867.78
#8 11.29 790.44