Noi/NOTES/2012-8-1
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WGBS sequencing library preparation using KAPA protocol (Eppendorf) and epMotion (Repeat!)
- The step of library sequencing prep will be filled soon.
File:ZhangLab 2 2012-08-02 11hr 31min-HAPMAP-bis-test.jpg
- Purified sequencing libraries with 0.8x vol AMPure bead with epMotion (3 strips), eluted with 25ul EB for each well -> total volume at the end ~70ul
File:ZhangLab 2 2012-08-02 16hr 05min bead-HAPMAP-WGBS.jpg
PAGE quantification of 400-600bp fragments
- Perform PAGE size-selection of the size range 400-600bp for a small scale sequencing
File:ZhangLab 2 2012-08-02 16hr 05min bead-HAPMAP-WGBS-400-600bp PQ.jpg
Lab IDs | Final conc. of 400-600bp (ng/ul) |
Total yields in 70ul (ng) |
#1 | 13.50 | 945.28 |
#2 | 9.66 | 675.94 |
#3 | 11.24 | 786.94 |
#4 | 13.64 | 955.09 |
#5 | 13.85 | 969.55 |
#6 | 8.95 | 626.85 |
#7 | 12.40 | 867.78 |
#8 | 11.29 | 790.44 |