Arichard:Protocols/MDA amplicon size selection

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MDA amplicon size selection

Prep

  • For each sample, place a 0.5 ml tube inside a 1.5 ml tube. Use a 20 G needle to punch a hole in the bottom of the 0.5 ml tube. UV treat.
  • Set up a nanosep tube for each sample.
  • UV treat 1 ml 100% EtOH, 40 ul 3 M NaAc, and 1 ul GlycoBlue in each 1.5 ml tube, 1 tube per sample.

Protocol

Size selection

Ethanol precipitation