Kun:LabNotes/ASE/2007-10-18

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Revision as of 01:25, 19 October 2007 by KunZhang (talk | contribs) (New page: ===Exp. 1 Perform PCR on CES22k and eSNP84k libraries to make MIP probes.=== Reaction CES22k(100nM): x 8 Template 0.2ul 1.6ul 10X buffe...)
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Exp. 1 Perform PCR on CES22k and eSNP84k libraries to make MIP probes.

Reaction CES22k(100nM):

                               x 8
    Template        0.2ul     1.6ul
    10X buffer       10ul      80ul
    10mM dNTP         2ul      16ul
    100uM pAP1V6U   0.4ul     3.2ul
    100uM phosAp2V6 0.4ul     3.2ul
    JumpStart Taq     2ul      16ul
    H2O              85ul     680ul

Reactions eSNP84k set1-5 (100nM):

                              x 8 x 5 
    Template        0.2ul      1.6ul x 5 
    10X buffer       10ul       80ul x 5 
    10mM dNTP         2ul       16ul x 5
    100uM AP1V41U   0.4ul      3.2ul x 5
    100uM Ap2V4     0.4ul      3.2ul x 5
    JumpStart Taq     2ul       16ul x 5
    H2O              85ul      680ul x 5

94C3min -> 20 cycles of (94C 45sec -> 58C 2min -> 72C 1min) -> 72C 3min -> 4C hold