Arichard:Reagents/celseq primers

From ZhangLabWiki
Revision as of 17:17, 14 February 2013 by >Andrew (Created page with "==CEL-Seq RT primer layout== ===Stock layout=== * Barcoded RT primers are in 48 wells of a 92 well plate at 200 uM each, in the following layout: {| {{table}} | align="cent...")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

CEL-Seq RT primer layout

Stock layout

  • Barcoded RT primers are in 48 wells of a 92 well plate at 200 uM each, in the following layout:
Barcode number 1 2 3 4 5 6 7 8 9 10 11 12
A 1 2 3 4 5 6 7 8 9 10 11 12
B 13 14 15 16 17 18 19 20 21 22 23 24
C 25 26 27 28 29 30 31 32 33 34 35 36
D 37 38 39 40 41 42 43 44 45 46 47 48
E Empty Empty Empty Empty Empty Empty Empty Empty Empty Empty Empty Empty
F Empty Empty Empty Empty Empty Empty Empty Empty Empty Empty Empty Empty
G Empty Empty Empty Empty Empty Empty Empty Empty Empty Empty Empty Empty
H Empty Empty Empty Empty Empty Empty Empty Empty Empty Empty Empty Empty
  • Serial dilutions are necessary to dilute the RT primers to the correct concentration.

Diluted primer layout