Noi/NOTES/2013-2-27

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Plan for probe preparation for Matt's project

  • I will be preparing probes using the same method as I did for LC Sciences oligoes, [[1]]
  • Check reagents to make sure I have all reagents ready for probe prep and padlock capture
Probe prep
- Waiting for seed oligos: expected to come next Wednesday (March6, 2013)
- eMIP_CA1_F and eMIP_CA1_R primer for expansion PCR and production PCR (very little left, ordered 2/28/2013)
- 2x KAPA SYBG FAST qPCR MM
- Nt.AlwI (NEB) and Nb.BsrDI (NEB) -> ordered 2/26/2013
- Qiaquick columns, TBU gel, Nanosep columns
Padlock capture
Note: There is no gap for this capture, so only need Ampligase
- Ampligase and buffer
- ExoI/ExoIII
- Amplification primers (Matt)