Noi/NOTES/2013-3-14

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Revision as of 18:59, 15 March 2013 by >Noi
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Set 1

Components 1rxn 6.5 rxn
Captured template 12.00 0.00
10uM Forward no barcodes 2.00 13.00
10uM Reverse +Indx 2.00 0.00
2x KAPA SYBG fast MM 50.00 325.00
H2O 34.00 221.00
Total 100.00 650.00
  • Aliquot 86ul, add 2ul of reverse Ind, 12ul of circular DNA

Set 2

Components ' '
Captured template 1rxn 6.5 rxn
10uM Forward + Indx 12.00 0.00
100uM Reverse no barcodes 2.00 0.00
2x KAPA SYBG fast MM 2.00 13.00
H2O 50.00 325.00
Total 34.00 221.00
100.00 650.00
  • Aliquot 86ul, add 2ul of Forward Ind, 12ul of circular DNA

File:ZhangLab 2 2013-03-15 11hr 27min SeqLib Insitu.jpg