Revision as of 18:59, 15 March 2013 by >Noi
Set 1
Components
|
1rxn
|
6.5 rxn
|
Captured template |
12.00 |
0.00
|
10uM Forward no barcodes |
2.00 |
13.00
|
10uM Reverse +Indx |
2.00 |
0.00
|
2x KAPA SYBG fast MM |
50.00 |
325.00
|
H2O |
34.00 |
221.00
|
Total |
100.00 |
650.00
|
- Aliquot 86ul, add 2ul of reverse Ind, 12ul of circular DNA
Set 2
Components
|
'
|
'
|
Captured template |
1rxn |
6.5 rxn
|
10uM Forward + Indx |
12.00 |
0.00
|
100uM Reverse no barcodes |
2.00 |
0.00
|
2x KAPA SYBG fast MM |
2.00 |
13.00
|
H2O |
50.00 |
325.00
|
Total |
34.00 |
221.00
|
|
100.00 |
650.00
|
- Aliquot 86ul, add 2ul of Forward Ind, 12ul of circular DNA
File:ZhangLab 2 2013-03-15 11hr 27min SeqLib Insitu.jpg