Noi/NOTES/2013-3-20

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Revision as of 22:33, 20 March 2013 by >Noi (Created page with "* http://genome-tech.ucsd.edu/LabNotes/index.php/noi:DMR220k_LabNotes '''Link to calendar''' * Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Noi/NOTES/201...")
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Skin Microbiom Project

  • Sequencing libraries: NP-SkinMB_N2_Ind17-88_Feb07.2013
  • Chris selected the top 20 libraries from analysis he did on light sequencing from the previous run (HL144, HL145) for Hiseq run.
  • I pooled equimolar of these libraries (~100ng/library) and do PAGE size-selection (300-600bp) as I did in the previous pool.
Index Well ID Conc. (ng/ul) Volume for 100ng (ul)
24 H3 8.37 11.95
34 B5 29.56 3.38
36 D5 30.58 3.27
37 E5 29.43 3.40
44 D6 13.98 7.15
48 H6 4.43 22.57
54 F7 16.68 6.00
55 G7 13.04 7.67
56 H7 9.28 10.78
58 B8 9.28 10.78
59 C8 10.88 9.19
66 B9 7.70 12.99
73 A10 4.78 20.92
74 B10 10.83 9.23
76 D10 13.33 7.50
78 F10 10.81 9.25
80 H10 6.92 14.45
84 D11 12.25 8.16
86 F11 11.91 8.40
88 H11 9.89 10.11