Tina:Cardiomyocyte differentiation protocols

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Monolayer-directed cardiomyocyte differentiation protocol

Christian Metallo Lab Protocol

1. Prepare cells on Matrigel-coated plates. Wait until cells reach 100% confluency.

2. [Day 0]

Aspirate culture medium. Add 2 ml RPMI/B27-A medium per well of a 12-well plate (or 1 ml medium per well of a 24-well plate). Add CHIR99021 stock into RPMI/B27-A medium (final conc. 12 uM CHIR99021).

3. [Day 1] Each day is an exact 24 hrs period.

Aspirate medium. Add 2 ml RPMI/B27-A medium per well of a 12-well plate. No CHIR99021 required.

4. [Day 2]

Add DMK stock into culture medium (final conc. 0.5 mM DMK). Do not change the used RPMI/B27-A medium.