Tina:Cardiomyocyte differentiation in H9 ESCs (CDH9)

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Revision as of 00:38, 11 April 2013 by >TinaLo
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  • Purpose: To determine how Metallo Lab's modified cardiomyocyte differentiation protocol works on H9 ESCs.
  • Cells: Brainbow H9 constructs

Experiment #1 (CDH9_E1_T1)

  • Study Purpose: Try 3 different induction medium and 2 different cell density during differentiation and see how it works on H9 ESCs directed cardiomyocyte differentiation.
  • Date: 4/10/2013 ~
  • Cells: H9 ESC line, Brainbow Clone #3 (H9Bb3)
  • Protocol used: Christian Metallo Lab protocol.
  • Three induction medium used: Gln+ induction medium,DMK induction medium
  • Two cell density used: 100%, 80% confluency.
  • Experimental design:

12-well plate #1

100% confluency 1 2 3 4
A Gln, repeat 1 Gln, repeat 2 Gln, repeat 3 Gln, repeat 4
C DMK, repeat 1 DMK, repeat 2 DMK, repeat 3 DMK, repeat 4
B Negative ctrl, repeat 1 Negative ctrl, repeat 2 Negative ctrl, repeat 3 Negative ctrl, repeat 4

12-well plate #2

80% confluency 1 2 3 4
A Gln, repeat 1 Gln, repeat 2 Gln, repeat 3 Gln, repeat 4
C DMK, repeat 1 DMK, repeat 2 DMK, repeat 3 DMK, repeat 4
B Negative ctrl, repeat 1 Negative ctrl, repeat 2 Negative ctrl, repeat 3 Negative ctrl, repeat 4
  • Cells seeded at 4/10. (1:6 dilution from the second passage of H9 ESCs under a feeder free culture system) OP9 cells were used as a negative control.