Tina:Cardiomyocyte differentiation in H9 ESCs (CDH9)
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- Purpose: To determine how Metallo Lab's modified cardiomyocyte differentiation protocol works on H9 ESCs.
- Cells: Brainbow H9 constructs
Experiment #1 (CDH9_E1_T1)
- Study Purpose: Try 3 different induction medium and 2 different cell density during differentiation and see how it works on H9 ESCs directed cardiomyocyte differentiation.
- Date: 4/10/2013 ~
- Cells: H9 ESC line, Brainbow Clone #3 (H9Bb3)
- Protocol used: Christian Metallo Lab protocol.
- Three induction medium used: Gln+ induction medium,DMK induction medium
- Two cell density used: 100%, 80% confluency.
- Experimental design:
12-well plate #1
100% confluency | 1 | 2 | 3 | 4 |
A | Gln, repeat 1 | Gln, repeat 2 | Gln, repeat 3 | Gln, repeat 4 |
C | DMK, repeat 1 | DMK, repeat 2 | DMK, repeat 3 | DMK, repeat 4 |
B | Negative ctrl, repeat 1 | Negative ctrl, repeat 2 | Negative ctrl, repeat 3 | Negative ctrl, repeat 4 |
12-well plate #2
80% confluency | 1 | 2 | 3 | 4 |
A | Gln, repeat 1 | Gln, repeat 2 | Gln, repeat 3 | Gln, repeat 4 |
C | DMK, repeat 1 | DMK, repeat 2 | DMK, repeat 3 | DMK, repeat 4 |
B | Negative ctrl, repeat 1 | Negative ctrl, repeat 2 | Negative ctrl, repeat 3 | Negative ctrl, repeat 4 |
- Cells seeded at 4/10. (1:6 dilution from the second passage of H9 ESCs under a feeder free culture system) OP9 cells were used as a negative control.