Daniel:Protocols/EtOHPrecip

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Revision as of 23:25, 17 April 2013 by >Djacobse
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Ethanol Precipitation

 Purpose:  To purifiy DNA.

Protocol

 1. Add (X=volume sample):
   At least 2.5X 100% Ethanol
   0.1X 3M NaOAc
   1/300X glycoblue (co-precipitate, helps you see the DNA)
 2. Incubate for at least 1 hour at -80 C
      For smaller fragments it is helpful to incubate overnight
 3. Centrifuge at 4 C
 4. Remove supernatant and add 500 uL chilled 75% EtOH
 5. Spin at 10000 rpm for 10 minutes at 4 C
 6. Dry the pellet in the hood 
 7. Resuspend pellet in desired volume ddH20
 8. Store DNA at -20C