Daniel:Protocols/Qiaquick Column
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Qiaquick Column Purification
Qiagen: Catalog #28104 (50) or #28106 (250)
Protocol
1. Add 5X PB (binding buffer) to sample 2. Load sample onto column and spin for 1 minute at 14000 rpm; discard flow through 3. Add 750 uL PE (wash buffer) to column and spin for 1 minute at 14000 rpm; discard flow through 4. Dry spin column for 1 minute at 14000 rpm; discard flow through 5. Let stand with cover open in fume hood for ~5 minutes 6. Transfer column to a new 1.5 mL eppendorf tube 7. Add ≥30 uL EB to column 8. Let stand 1 minute 9. Spin for 1 minute at 14000 rpm