Revision as of 21:19, 25 April 2013 by >Djacobse
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DpnII Digest (Continued from 4/24/2013)
1. Took samples from thermocycler
2. Purified using Qiagen column
3. Set up following TBE gel
Sample
|
Ladder
|
V4S3A-Pre Digestion
|
V4S3B-Pre Digestion
|
V4S3A-Post Digestion
|
V4S3A-Post Column
|
V4S3B-Post Column
|
Ladder
|
V6S3A-Pre Digestion
|
V6S3B-Pre Digestion
|
V6S3A-Post Digestion
|
V6S3A-Post Column
|
V6S3B-Post Column
|
uL sample
|
1
|
2
|
2
|
2
|
2
|
2
|
1
|
2
|
2
|
2
|
2
|
2
|
uL buffer
|
5
|
4
|
4
|
4
|
4
|
4
|
5
|
4
|
4
|
4
|
4
|
4
|
uL dye
|
2
|
2
|
2
|
2
|
2
|
2
|
2
|
2
|
2
|
2
|
2
|
2
|
loaded
|
7
|
7
|
7
|
7
|
7
|
7
|
7
|
7
|
7
|
7
|
7
|
7
|
4. Ran gel for 30 minutes at 200 V.
5. Stained for 10 minutes with 3 uL SYBR gold
6. Measured concentration in Nanodrop
Nanodrop Results
Sample
|
ng/uL
|
uL
|
ng
|
V4S3A
|
102.2
|
30
|
3066
|
V4S3B
|
86.8
|
30
|
2604
|
V6S3A
|
80.1
|
30
|
2403
|
V6S3B
|
77.9
|
30
|
2337
|
Gel Results
File:DpnII Digest 042513.jpg