Daniel:Notebook/HiResChrPaint/2013-4-1

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Revision as of 00:05, 30 April 2013 by >Djacobse (Created page with "=Probe Prep (Started 4/26/13)= Back to Calendar ==DpnII Digestion== Purpose: Trying to erase ...")
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Probe Prep (Started 4/26/13)

Back to Calendar

DpnII Digestion

Purpose: Trying to erase strange black marks from previous gels
1. Set up reactions according to table
Probe ng/uL uL 10x Buffer DpnII H2O Total
V4S1 41.6 30 5 5 10 50
V4S2 159.8 30 5 5 10 50
V4S3 73.1 30 5 5 10 50
V4S4 47.8 30 5 5 10 50
V6S1 84.4 30 5 5 10 50
V6S2 111.9 30 5 5 10 50
V6S3 131.4 30 5 5 10 50
V6S4 106.9 30 5 5 10 50
2. Incubate at 37 C for 1 hr; 20 minute heat shock at 65C
3. Column purification, elute with 30 uL
4. Measure concentration in Nanodrop