Hosuk:LabNotes/2013-5-2

From ZhangLabWiki
Revision as of 22:38, 2 May 2013 by >Hosuki78
Jump to navigation Jump to search

Result - RT check

  • based on Debugging plan (05/01)


  • RT process seemed work.


  • +RTase sample showed very strong Cy5 signal which is supposed cDNA
  • comparing to -RTase result, the bright singal over the cell seemed they were cDNAs
  • -RTase sample has very low intensity of signals in the cell, and
  • not many spots were observed, which spots were outside of cells.


  • Most of signal came from cell structure.


  • The picture of -RTase is the position where the most number of spots were observed in all over the area.
  • In the +RTase sample, similar number or density of spots were observed in all over the area.
  • I don't know what are the large spots. Are they also cDNAs?


  • +RTase
S2:+RTase, Brighfield, 20x S2:+RTase, Cy5, 20x S2:+RTase, Merged
(Original) (Original)
File:RTCheck +RTase fig08 20xobj pos04 BF exp0.2-EM00.jpg File:RTCheck +RTase fig07 20xobj pos04 Cy5 exp0.2-EM40.jpg File:Composite +RTase fig07-08.jpg


  • -RTase (No RTase)
S3:-RTase, Brighfield, 20x S3:-RTase, Cy5, 20x S3:-RTase, Merged
(Original) (Original)
File:RTCheckNoRTase fig08 20xobj pos05 BF exp0.2-EM00.jpg File:RTCheckNoRTase fig07 20xobj pos04 Cy5 exp0.2-EM40.jpg File:Composite NoRTase fig07-08.jpg