Daniel:Notebook/HiResChrPaint/2013-5-11

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Revision as of 00:27, 12 May 2013 by >Djacobse (Created page with "=Probe Prep Test 2 (Started 5/10/2013)= Back to Calendar ==Ethanol Precipitation== 7. Centrif...")
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Probe Prep Test 2 (Started 5/10/2013)

Back to Calendar

Ethanol Precipitation

7. Centrifuged 10000 rpm for 30 minutes at 4C
8. Removed supernatant added 750 uL 75% EtOH
9. Centrifuged 10000 rpm for 12 minutes at 4C
10. Removed supernatant, dried in hood
11. Resuspended with 50 uL
12. Column Purification, eluted with 50 uL
13. measured concentration in nanodrop

Nanodrop Results

DpnII Digestion

Info

1. Prepared samples according to table