Revision as of 21:27, 4 June 2013 by >Djacobse
Back to Calendar
DpnII Digest (from 6/3/2013)
4. Column Purification, elute with 30 uL
5. Measure concentrations in Nanodrop
Nanodrop Results
Sample
|
ng/uL
|
ug in 30 uL
|
Yield (%)
|
alpha-4B
|
127.1
|
3.8
|
80.9
|
alpha-6B
|
122.4
|
3.7
|
78.6
|
beta-4B
|
122.6
|
3.7
|
83.6
|
beta-6B
|
112.5
|
3.4
|
73.3
|
TBE Gel
1. Gel setup
Lane
|
1
|
2
|
3
|
4
|
5
|
6
|
7
|
8
|
9
|
10
|
11
|
12
|
Sample
|
10 bp ladder
|
V4S2-B**
|
V4S2-A
|
V6S2-A
|
V4S2-A*
|
V4S2-B*
|
10 bp ladder
|
V6S2-B**
|
V4S2-A
|
V6S2-A
|
V4S2-A*
|
V4S2-B*
|
2. Ran gel for 25 minutes at 250 V
3. Stained with 3 uL SYBR gold for 3 minutes
Gel Image