Matt:LabNotes/2013-6-4

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Revision as of 23:18, 4 June 2013 by >Mzcai (Created page with "Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-22 ===Probe Capture=== *Use the ~37.4 pmole of probes from [http://genome-tech.ucsd.edu/La...")
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Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-22

Probe Capture

  • Use the ~37.4 pmole of probes from [1] and [2] to produce maximum number of circularized probes with U's incorporated for Ho Suk's experiments
  • Use gDNA as template/target
  • Use a probe:target ratio of 100:1

100:1 Probe:Target Calculations

56 ug of gDNA 140 ul of Probes