Matt:LabNotes/2013-6-4
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Continued from: http://genome-tech.ucsd.edu/LabNotes/index.php/Matt:LabNotes/2013-5-22
Probe Capture
- Use the ~37.4 pmole of probes from [1] and [2] to produce maximum number of circularized probes with U's incorporated for Ho Suk's experiments
- Use gDNA as template/target
- Use a probe:target ratio of 100:1
100:1 Probe:Target Calculations
56 ug of gDNA 140 ul of Probes