Daniel:Notebook/HiResChrPaint/2013-6-10

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Revision as of 17:24, 19 June 2013 by >Djacobse
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Probe Prep 3 (Started 5/29/2013)

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TBU Gel Quantification

Protocol 2

1. Sample matrix
Lane 2 3 4 5 6 7 8 9 10 11
Sample Low Mass Ladder Low Mass Ladder V4S2 V6S2 V4S2* V6S2* V4S2 V6S2 V4S2* V6S2*
uL sample 0.5 1 0.5 0.5 0.5 0.5 2** 2** 2** 2**
uL TBE 1.5 1 9.5 9.5 9.5 9.5 1 1 1 1
uL 2X Urea Buffer 2 2 10 10 10 10 1 1 1 1
Dilution NA NA 1:10 1:10 1:10 1:10 1:20 1:20 1:20 1:20
Loaded 4 4 4 4 4 4 4 4 4 4
2. Ran gel for 18 minutes at 250 V
3. Stained for 25 minutes with 3 uL SYBR Gold

Results

Volume Report: File:GelQuant 061013.xls

File:GelQuant 061013.jpg

File:GelQuant 051513 Volume.jpg

Results

Sample ng/uL ug in 10 uL
V4S2 119.9 1.2
V6S2 122.9 1.2
V4S2* 158.7 1.6
V6S2* 153.4 1.5

Note: *denotes no aa-dUTP