Hosuk:LabNotes/2013-6-12
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Day 3
- Jay finished last step, washing RCA and binding Cy3 probe, and image
- Rolonies are pretty well generated inside cells,
- and the signal is very strong, so there were almost no background
- Even though there were some of debris, they could be come from fixing step.
- No RCA signal were in -RT sample
20x, +RT vs. -RT (1 wash)
Raw images : +RT, -RT |
File:20x 1wash.png |
20x, +RT, NA=0.4 vs. 0.8 (2 wash)
Raw images : NA=0.4, NA=0.8 |
File:20x 2wash 0.4NA 0.8NA.png |