Hosuk:LabNotesMDA/2013-6-28

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Revision as of 17:51, 28 June 2013 by >Hosuki78 (→‎Result)
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MDA with PGP1F, 6th

  • MDA run for 8hr @06/27
  • 400mM ALS --> original protocol
  • Slow mixing --> 1 sec period, mixing for 5min
  • Run MDA in ring mixer --> 25th chamber!
  • Push ring mixer volume with air
    • Left 8 chamber were empty, too many empty chamber...


Result

  • qPCR result looked good.
  • Most of chambers have 500pg~2400pg of amplicons.
  • Left 8 chamber group were nearly empty, so that's why ch6 has almost no amplicon was detected.
  • 25th chamber(Ring mixer) had less amount than other chamber did, but there was amplicon!
  • It seemed that low conc. of ALS doesn’t work in the MDA device… I’ll try higher conc. than 133mM, such as 250mM ALS.


File:QPCR Result 2013-06-28 Edited.png File:QPCR Result MassTable 2013-06-28.png

CoRE fragmentation

  • Done @06/28