Blue:RNAseq Primer List

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Adaptor Structure

Rui's totoRNAseq Page


totoRNA 5' primer sequence

  • The 5' TSO adaptors list
Tube ID Primer full name Primer full sequence PE_F-N10 Barcode TSO
TSO.r04 iso_B_TSO_N10 iso-dCiso-dGiso-dCACACTCTTTCCCTACACGACGNNNUNNNNUNNNrGrGrG /5Me-isodC//iisodG//iMe-isodC/ACACTCTTTCCCTACACGACGNNN/ideoxyU/NNNN/ideoxyU/NNN \ rGrGrG
TSO.r05 IsoL_B_TSO_N8 T-iSp18-ACACTCTTTCCCTACACGACGNNNNUNNNNrGrG+G T/iSp18/ACACTCTTTCCCTACACGACGNNNN/ideoxyU/NNNNrGrG+G \ rGrG+G
TSO.r04_N6 iso_B_TSO_N6 isodC-isodG-isodC-ACACTCTTTCCCTACACGACGNNNUNNNrGrGrG /5Me-isodC//iisodG//iMe-isodC/ACACTCTTTCCCTACACGACGNNN/ideoxyU/NNNrGrGrG \ rGrGrG
TSO.r06 iso_SMARTer II A isodC-isodG-isodC-AAGCAGTGGTATCAACGCAGAGTACrGrGrG /5Me-isodC//iisodG//iMe-isodC/AAGCAGTGGTATCAACGCAGAGTACrGrGrG \ rGrGrG
PB_PCR.F PB_PCR.F AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG \
PB_PCR.F* PB_PCR.Fmod AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG A*A*T*GATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACG \
P1-STRT P1-STRT AATGATACGGCGACCACCGAGATCTAAGCAGTGGTATCAACGCAGAGT \
ILMN_PCR.F* ILMN_PCR.Fmod AATGATACGGCGACCACCGAGATC A*A*T*GATACGGCGACCACCGAGATC \

totoRNA 3' primer sequence

  • The 3' T20 adaptors list
Tube ID Primer full name Primer full sequence PE_R Barcode T20V
T20VN.r01 T20VN_PE_R /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTVN /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT TTTTTTTTTTTTTTTTTTTTVN
T20V.id01 T20V.BC8.PE_R.Indx1 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCTGAGACTTTTTTTTTTTTTTTTTTTTV /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCT TCTGAGAC TTTTTTTTTTTTTTTTTTTTV
T20V.id02 T20V.BC8.PE_R.Indx2 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGCCCAAGTTTTTTTTTTTTTTTTTTTTV TGCCCAAG
T20V.id03 T20V.BC8.PE_R.Indx3 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGACTCGCTTTTTTTTTTTTTTTTTTTTTV GACTCGCT
T20V.id04 T20V.BC8.PE_R.Indx4 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCACTCCATTTTTTTTTTTTTTTTTTTTV CCACTCCA
T20V.id05 T20V.BC8.PE_R.Indx5 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAACAGGGTTTTTTTTTTTTTTTTTTTTV GAACAGGG
T20V.id06 T20V.BC8.PE_R.Indx6 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTGCTGATTTTTTTTTTTTTTTTTTTTTV CTGCTGAT
T20V.id07 T20V.BC8.PE_R.Indx7 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTAGTATCTTTTTTTTTTTTTTTTTTTTV CTAGTATC
T20V.id08 T20V.BC8.PE_R.Indx8 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGCTGAAATTTTTTTTTTTTTTTTTTTTV GGCTGAAA
T20V.id09 T20V.BC8.PE_R.Indx9 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAGTGCTTTTTTTTTTTTTTTTTTTTTTV GAGTGCTT
T20V.id10 T20V.BC8.PE_R.Indx10 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATACGCCATTTTTTTTTTTTTTTTTTTTV ATACGCCA
T20V.id11 T20V.BC8.PE_R.Indx11 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATGCGTTATTTTTTTTTTTTTTTTTTTTV ATGCGTTA
T20V.id12 T20V.BC8.PE_R.Indx12 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCACCCTTTTTTTTTTTTTTTTTTTTTTV TCACCCTT
T20V.id13 T20V.BC8.PE_R.Indx13 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCAACCGCTTTTTTTTTTTTTTTTTTTTV CCAACCGC
T20V.id14 T20V.BC8.PE_R.Indx14 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGATTACCTTTTTTTTTTTTTTTTTTTTV GGATTACC
T20V.id15 T20V.BC8.PE_R.Indx15 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAGAGACATTTTTTTTTTTTTTTTTTTTV CAGAGACA
T20V.id16 T20V.BC8.PE_R.Indx16 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAATAGTGTTTTTTTTTTTTTTTTTTTTV CAATAGTG
T20V.id17 T20V.BC8.PE_R.Indx17 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGTGGCCATTTTTTTTTTTTTTTTTTTTV GGTGGCCA
T20V.id18 T20V.BC8.PE_R.Indx18 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGAGGGAGTTTTTTTTTTTTTTTTTTTTV GGAGGGAG
T20V.id19 T20V.BC8.PE_R.Indx19 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTTGTCCTTTTTTTTTTTTTTTTTTTTTV GTTGTCCT
T20V.id20 T20V.BC8.PE_R.Indx20 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTCGACCTTTTTTTTTTTTTTTTTTTTTV TTCGACC
T20V.id21 T20V.BC8.PE_R.Indx21 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCTTATTGTTTTTTTTTTTTTTTTTTTTV GCTTATTG
T20V.id22 T20V.BC8.PE_R.Indx22 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAACCTTTTTTTTTTTTTTTTTTTTTTTV CAACCTTT
T20V.id23 T20V.BC8.PE_R.Indx23 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTTAGGTATTTTTTTTTTTTTTTTTTTTV CTTAGGTA
T20V.id24 T20V.BC8.PE_R.Indx24 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCTCACCATTTTTTTTTTTTTTTTTTTTV GCTCACCA
T20V.id25 T20V.BC8.PE_R.Indx25 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGAAATGCTTTTTTTTTTTTTTTTTTTTV AGAAATGC
T20V.id26 T20V.BC8.PE_R.Indx26 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTTACTGATTTTTTTTTTTTTTTTTTTTV TACTGATT
T20V.id27 T20V.BC8.PE_R.Indx27 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTCACGGGTTTTTTTTTTTTTTTTTTTTV CTCACGGG
T20V.id28 T20V.BC8.PE_R.Indx28 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCACTGTCATTTTTTTTTTTTTTTTTTTTV
T20V.id29 T20V.BC8.PE_R.Indx29 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTCTTGGGTTTTTTTTTTTTTTTTTTTTV
T20V.id30 T20V.BC8.PE_R.Indx30 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTACCGGCTTTTTTTTTTTTTTTTTTTTTTV
T20V.id31 T20V.BC8.PE_R.Indx31 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTAACCACATTTTTTTTTTTTTTTTTTTTV
T20V.id32 T20V.BC8.PE_R.Indx32 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTACGCGCGTTTTTTTTTTTTTTTTTTTTV
T20V.id33 T20V.BC8.PE_R.Indx33 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCTCGATGTTTTTTTTTTTTTTTTTTTTV
T20V.id34 T20V.BC8.PE_R.Indx34 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGAGCTCATTTTTTTTTTTTTTTTTTTTV
T20V.id35 T20V.BC8.PE_R.Indx35 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTATGGTCCTTTTTTTTTTTTTTTTTTTTV
T20V.id36 T20V.BC8.PE_R.Indx36 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGTCCGGCTTTTTTTTTTTTTTTTTTTTV
T20V.id37 T20V.BC8.PE_R.Indx37 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAAGGAATCTTTTTTTTTTTTTTTTTTTTV
T20V.id38 T20V.BC8.PE_R.Indx38 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCATCGTAGTTTTTTTTTTTTTTTTTTTTV
T20V.id39 T20V.BC8.PE_R.Indx39 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTGTTGAGTTTTTTTTTTTTTTTTTTTTV
T20V.id40 T20V.BC8.PE_R.Indx40 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATGTATCCTTTTTTTTTTTTTTTTTTTTV
T20V.id41 T20V.BC8.PE_R.Indx41 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGACAGGTTTTTTTTTTTTTTTTTTTTTV
T20V.id42 T20V.BC8.PE_R.Indx42 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTACGCCAATTTTTTTTTTTTTTTTTTTTV
T20V.id43 T20V.BC8.PE_R.Indx43 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAAGGCAATTTTTTTTTTTTTTTTTTTTV
T20V.id44 T20V.BC8.PE_R.Indx44 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCACCCGAGTTTTTTTTTTTTTTTTTTTTV
T20V.id45 T20V.BC8.PE_R.Indx45 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGTGTGTCTTTTTTTTTTTTTTTTTTTTV
T20V.id46 T20V.BC8.PE_R.Indx46 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCTTGTGCTTTTTTTTTTTTTTTTTTTTV
T20V.id47 T20V.BC8.PE_R.Indx47 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGTACCTGTTTTTTTTTTTTTTTTTTTTV
T20V.id48 T20V.BC8.PE_R.Indx48 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGCGTCCTTTTTTTTTTTTTTTTTTTTTV
T20V.id49 T20V.BC8.PE_R.Indx49 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGAACTGTTTTTTTTTTTTTTTTTTTTTV
T20V.id50 T20V.BC8.PE_R.Indx50 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCAATGCTCTTTTTTTTTTTTTTTTTTTTV
T20V.id51 T20V.BC8.PE_R.Indx51 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTTAGGCGTTTTTTTTTTTTTTTTTTTTV
T20V.id52 T20V.BC8.PE_R.Indx52 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTAGACGCTTTTTTTTTTTTTTTTTTTTTV
T20V.id53 T20V.BC8.PE_R.Indx53 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCTGGTGTGTTTTTTTTTTTTTTTTTTTTV
T20V.id54 T20V.BC8.PE_R.Indx54 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGCGGTAATTTTTTTTTTTTTTTTTTTTV
T20V.id55 T20V.BC8.PE_R.Indx55 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCTTAAGTTTTTTTTTTTTTTTTTTTTTV
T20V.id56 T20V.BC8.PE_R.Indx56 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCGGTTTCTTTTTTTTTTTTTTTTTTTTV
T20V.id57 T20V.BC8.PE_R.Indx57 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAAGCCTAGTTTTTTTTTTTTTTTTTTTTV
T20V.id58 T20V.BC8.PE_R.Indx58 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTAGCCAGGTTTTTTTTTTTTTTTTTTTTV
T20V.id59 T20V.BC8.PE_R.Indx59 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTGGTAAATTTTTTTTTTTTTTTTTTTTV
T20V.id60 T20V.BC8.PE_R.Indx60 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCACATAGGTTTTTTTTTTTTTTTTTTTTV
T20V.id61 T20V.BC8.PE_R.Indx61 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCCGGTGATTTTTTTTTTTTTTTTTTTTV
T20V.id62 T20V.BC8.PE_R.Indx62 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCTAAACCTTTTTTTTTTTTTTTTTTTTV
T20V.id63 T20V.BC8.PE_R.Indx63 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCGTGTCCCTTTTTTTTTTTTTTTTTTTTV
T20V.id64 T20V.BC8.PE_R.Indx64 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGGGTAGGTTTTTTTTTTTTTTTTTTTTV
T20V.id65 T20V.BC8.PE_R.Indx65 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGGAGATGTTTTTTTTTTTTTTTTTTTTV
T20V.id66 T20V.BC8.PE_R.Indx66 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGGAAGTATTTTTTTTTTTTTTTTTTTTV
T20V.id67 T20V.BC8.PE_R.Indx67 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGGCATGCTTTTTTTTTTTTTTTTTTTTV
T20V.id68 T20V.BC8.PE_R.Indx68 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTAGGTACTTTTTTTTTTTTTTTTTTTTV
T20V.id69 T20V.BC8.PE_R.Indx69 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCTGGATTTTTTTTTTTTTTTTTTTTTTV
T20V.id70 T20V.BC8.PE_R.Indx70 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGCTCCCTTTTTTTTTTTTTTTTTTTTTV
T20V.id71 T20V.BC8.PE_R.Indx71 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAAGCGCCCTTTTTTTTTTTTTTTTTTTTV
T20V.id72 T20V.BC8.PE_R.Indx72 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCGACGGATTTTTTTTTTTTTTTTTTTTTV
T20V.id73 T20V.BC8.PE_R.Indx73 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGGATAGCGTTTTTTTTTTTTTTTTTTTTV
T20V.id74 T20V.BC8.PE_R.Indx74 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGCCCAGTGTTTTTTTTTTTTTTTTTTTTV
T20V.id75 T20V.BC8.PE_R.Indx75 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTGAAGACTTTTTTTTTTTTTTTTTTTTV
T20V.id76 T20V.BC8.PE_R.Indx76 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAATCAGGATTTTTTTTTTTTTTTTTTTTV
T20V.id77 T20V.BC8.PE_R.Indx77 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATGCTTCTTTTTTTTTTTTTTTTTTTTTV
T20V.id78 T20V.BC8.PE_R.Indx78 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCAAGCAGTTTTTTTTTTTTTTTTTTTTV
T20V.id79 T20V.BC8.PE_R.Indx79 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAAGTCGTTTTTTTTTTTTTTTTTTTTTV
T20V.id80 T20V.BC8.PE_R.Indx80 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCGCCGCATTTTTTTTTTTTTTTTTTTTV
T20V.id81 T20V.BC8.PE_R.Indx81 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTCAGTTACTTTTTTTTTTTTTTTTTTTTV
T20V.id82 T20V.BC8.PE_R.Indx82 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTAACGTGCTTTTTTTTTTTTTTTTTTTTV
T20V.id83 T20V.BC8.PE_R.Indx83 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGTAGGATTTTTTTTTTTTTTTTTTTTTV
T20V.id84 T20V.BC8.PE_R.Indx84 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTAGCACATCTTTTTTTTTTTTTTTTTTTTV
T20V.id85 T20V.BC8.PE_R.Indx85 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTGTCTTGTTTTTTTTTTTTTTTTTTTTV
T20V.id86 T20V.BC8.PE_R.Indx86 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTGCAACCATTTTTTTTTTTTTTTTTTTTV
T20V.id87 T20V.BC8.PE_R.Indx87 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCACTTGTCTTTTTTTTTTTTTTTTTTTTV
T20V.id88 T20V.BC8.PE_R.Indx88 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAGTTTGGTTTTTTTTTTTTTTTTTTTTV
T20V.id89 T20V.BC8.PE_R.Indx89 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGTGCTGGATTTTTTTTTTTTTTTTTTTTV
T20V.id90 T20V.BC8.PE_R.Indx90 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTGAGAGCGCTTTTTTTTTTTTTTTTTTTTV
T20V.id91 T20V.BC8.PE_R.Indx91 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCGAACATTTTTTTTTTTTTTTTTTTTTV
T20V.id92 T20V.BC8.PE_R.Indx92 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTACTATGCGTTTTTTTTTTTTTTTTTTTTV
T20V.id93 T20V.BC8.PE_R.Indx93 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTAGCACTTTTTTTTTTTTTTTTTTTTTV
T20V.id94 T20V.BC8.PE_R.Indx94 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTATGTCCATTTTTTTTTTTTTTTTTTTTTV
T20V.id95 T20V.BC8.PE_R.Indx95 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTCCCTGCCTTTTTTTTTTTTTTTTTTTTTV
T20V.id96 T20V.BC8.PE_R.Indx96 /5Biosg/GCATTCCTGCTGAACCGCTCTTCCGATCTTTGTCAGCTTTTTTTTTTTTTTTTTTTTV
N2.id1 PCR_R.N2Ind1 CAAGCAGAAGACGGCATACGAGATCGTGATCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT CGTGAT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id2 PCR_R.N2Ind2 CAAGCAGAAGACGGCATACGAGATACATCGCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT ACATCG CTCGGCATTCCTGCTGAACCGCTCTT
N2.id3 PCR_R.N2Ind3 CAAGCAGAAGACGGCATACGAGATGCCTAACTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT GCCTAA CTCGGCATTCCTGCTGAACCGCTCTT
N2.id4 PCR_R.N2Ind4 CAAGCAGAAGACGGCATACGAGATTGGTCACTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT TGGTCA CTCGGCATTCCTGCTGAACCGCTCTT
N2.id5 PCR_R.N2Ind5 CAAGCAGAAGACGGCATACGAGATCACTGTCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT CACTGT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id6 PCR_R.N2Ind6 CAAGCAGAAGACGGCATACGAGATATTGGCCTCGGCATTCCTGCTGAACCGCTCT CAAGCAGAAGACGGCATACGAGAT ATTGGC CTCGGCATTCCTGCTGAACCGCTCTT
N2.id7 PCR_R.N2Ind7 CAAGCAGAAGACGGCATACGAGATGATCTGCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT GATCTG CTCGGCATTCCTGCTGAACCGCTCTT
N2.id8 PCR_R.N2Ind8 CAAGCAGAAGACGGCATACGAGATTCAAGTCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT TCAAGT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id9 PCR_R.N2Ind9 CAAGCAGAAGACGGCATACGAGATCTGATCCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT CTGATC CTCGGCATTCCTGCTGAACCGCTCTT
N2.id10 PCR_R.N2Ind10 CAAGCAGAAGACGGCATACGAGATAAGCTACTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT AAGCTA CTCGGCATTCCTGCTGAACCGCTCTT
N2.id11 PCR_R.N2Ind11 CAAGCAGAAGACGGCATACGAGATGTAGCCCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT GTAGCC CTCGGCATTCCTGCTGAACCGCTCTT
N2.id12 PCR_R.N2Ind12 CAAGCAGAAGACGGCATACGAGATTACAAGCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT TACAAG CTCGGCATTCCTGCTGAACCGCTCTT
N2.id13 PCR_R.N2Ind13 CAAGCAGAAGACGGCATACGAGAT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id14 PCR_R.N2Ind14 CAAGCAGAAGACGGCATACGAGAT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id81 PCR_R.N2Ind81 CAAGCAGAAGACGGCATACGAGATCCAGGTCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT CCAGGT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id82 PCR_R.N2Ind82 CAAGCAGAAGACGGCATACGAGATCGTTTGCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT CGTTTG CTCGGCATTCCTGCTGAACCGCTCTT
N2.id83 PCR_R.N2Ind83 CAAGCAGAAGACGGCATACGAGATGACCACCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT GACCAC CTCGGCATTCCTGCTGAACCGCTCTT
N2.id84 PCR_R.N2Ind84 CAAGCAGAAGACGGCATACGAGATACAAGACTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT ACAAGA CTCGGCATTCCTGCTGAACCGCTCTT
N2.id85 PCR_R.N2Ind85 CAAGCAGAAGACGGCATACGAGATACCGCACTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT ACCGCA CTCGGCATTCCTGCTGAACCGCTCTT
N2.id86 PCR_R.N2Ind86 CAAGCAGAAGACGGCATACGAGATTGGGTACTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT TGGGTA CTCGGCATTCCTGCTGAACCGCTCTT
N2.id87 PCR_R.N2Ind87 CAAGCAGAAGACGGCATACGAGATATTCCGCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT ATTCCG CTCGGCATTCCTGCTGAACCGCTCTT
N2.id88 PCR_R.N2Ind88 CAAGCAGAAGACGGCATACGAGATGAATGTCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT GAATGT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id89 PCR_R.N2Ind89 CAAGCAGAAGACGGCATACGAGATGCTGATCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT GCTGAT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id90 PCR_R.N2Ind90 CAAGCAGAAGACGGCATACGAGATAGTGCTCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT AGTGCT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id91 PCR_R.N2Ind91 CAAGCAGAAGACGGCATACGAGATCAGGGACTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT CAGGGA CTCGGCATTCCTGCTGAACCGCTCTT
N2.id92 PCR_R.N2Ind92 CAAGCAGAAGACGGCATACGAGATCATGCGCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT CATGCG CTCGGCATTCCTGCTGAACCGCTCTT
N2.id93 PCR_R.N2Ind93 CAAGCAGAAGACGGCATACGAGATTGCCTACTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT TGCCTA CTCGGCATTCCTGCTGAACCGCTCTT
N2.id94 PCR_R.N2Ind94 CAAGCAGAAGACGGCATACGAGATCTATACCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT CTATAC CTCGGCATTCCTGCTGAACCGCTCTT
N2.id95 PCR_R.N2Ind95 CAAGCAGAAGACGGCATACGAGATCCGAGTCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT CCGAGT CTCGGCATTCCTGCTGAACCGCTCTT
N2.id96 PCR_R.N2Ind96 CAAGCAGAAGACGGCATACGAGATACCTGCCTCGGCATTCCTGCTGAACCGCTCTT CAAGCAGAAGACGGCATACGAGAT ACCTGC CTCGGCATTCCTGCTGAACCGCTCTT
PGR_PCR_R CAAGCAGAAGACGGCATACGAGATCTCGGCATTCCTGCTGAACCGCTCTT CGAGAT
PGR_PCR_Rb PGR_PCR_Rmod CAAGCAGAAGACGGCATACGAGATCTCGGCATTCCTGCTGAACCGCTCTT /5Phos/CAAGCAGAAGACGGCATACGAGATCTCGGCATTCCTGCTGAACCGCTCTT
ILMN_PCR_Rb ILMN_PCR_Rmod CAAGCAGAAGACGGCATACGAGAT /5Phos/CAAGCAGAAGACGGCATACGAGAT

IVT 5' primer sequence

  • The 5' adaptors list
Primer full name Primer full sequence PB_PCR_F Complementary Seq Random Sequence
RL.IVT.N6 ACACTCTTTCCCTACACGACGNNNNNN ACACTCTTTCCCTACACGACG NNNNNN
RL.IVT.N9 ACACTCTTTCCCTACACGACGNNNNNNNNN ACACTCTTTCCCTACACGACG NNNNNNNNN


IVT 3' primer sequence

  • The 3' adaptors list
Primer full name Primer full sequence T20 Complementary Seq Novel T7 Seq
RL.Add.T7 CTTTAATTATGCTGAGTGATATCCCCGTAAGGACGACTTGGCGAGAA GCATTCCTGCTGAACCGCTCTT CTTTAATTATGCTGAGTGATATCCC


MARS-Seq/STRT-Seq/Smart-seq2 Primer Sequences

Protocol Primer full name Primer full sequence Modification Barcode Seq
MARS-Seq MARS_T20N_id01 CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTCGTGATNNNNTTTTTTTTTTTTTTTTTTTTN N/A
MARS-Seq MARS_T20N_id02 CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTACATCGNNNNTTTTTTTTTTTTTTTTTTTTN N/A
MARS-Seq MARS_T20N_id03 CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTGCCTAANNNNTTTTTTTTTTTTTTTTTTTTN N/A
MARS-Seq MARS_T20N_id04 CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTTGGTCANNNNTTTTTTTTTTTTTTTTTTTTN N/A
MARS-Seq MARS_T20N_id05 CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTCACTGTNNNNTTTTTTTTTTTTTTTTTTTTN N/A
MARS-Seq MARS_T20N_id06 CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTATTGGCNNNNTTTTTTTTTTTTTTTTTTTTN N/A
MARS-Seq MARS_T20N_id07 CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTGATCTGNNNNTTTTTTTTTTTTTTTTTTTTN N/A
MARS-Seq MARS_T20N_id08 CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTTCAAGTNNNNTTTTTTTTTTTTTTTTTTTTN N/A
MARS-Seq MARS_T20N_id09 CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTCTGATCNNNNTTTTTTTTTTTTTTTTTTTTN N/A
MARS-Seq MARS_T20N_id10 CGATTGAGGCCGGTAATACGACTCACTATAGGGGCGACGTGTGCTCTTCCGATCTAAGCTANNNNTTTTTTTTTTTTTTTTTTTTN N/A
MARS-Seq MARS_RT_2 TCTAGCCTTCTCGCAGCACATC N/A
MARS-Seq P5_Rd1_PCR_F AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT N/A
MARS-Seq P7_Rd2_PCR_R CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT N/A
MARS-Seq MARS_lig_NNNX4_ix1 /5Phos/GACTNNNAGATCGGAAGAGCGTCGTGTAG/3SpC3/ 5Phos/3SpC3 GACT
MARS-Seq MARS_lig_NNNX4_ix2 /5Phos/CATGNNNAGATCGGAAGAGCGTCGTGTAG/3SpC3/ 5Phos/3SpC3 CATG
MARS-Seq MARS_lig_NNNX4_ix3 /5Phos/CCAANNNAGATCGGAAGAGCGTCGTGTAG/3SpC3/ 5Phos/3SpC3 CCAA
MARS-Seq MARS_lig_NNNX4_ix4 /5Phos/CTGTNNNAGATCGGAAGAGCGTCGTGTAG/3SpC3/ 5Phos/3SpC3 CTGT
MARS-Seq MARS_lig_NNNX4_ix5 /5Phos/GTAGNNNAGATCGGAAGAGCGTCGTGTAG/3SpC3/ 5Phos/3SpC3 GTAG
MARS-Seq MARS_lig_NNNX4_ix6 /5Phos/TGATNNNAGATCGGAAGAGCGTCGTGTAG/3SpC3/ 5Phos/3SpC3 TGAT
Smart-seq2 Smart2-T30VN AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN N/A
Smart-seq2 Smart2_ISPCR AAGCAGTGGTATCAACGCAGAGT N/A
Smart-Seq2 Smart2-TSO AAGCAGTGGTATCAACGCAGAGTACATrGrG+G LNA (+G)
Quartz-Seq RT primer (WTA) TATAGAATTCGCGGCCGCTCGCGATAATACGACTCACTATAGGGCGTTTTTTTTTTTTTTTTTTTTTTTT N/A
Quartz-Seq Tagging primer TATAGAATTCGCGGCCGCTCGCGATTTTTTTTTTTTTTTTTTTTTTTT N/A
Quartz-Seq Suppression primer (NH2)-GTATAGAATTCGCGGCCGCTCGCGAT

Sequencing Primers

Primer full name Primer full sequence Protocol Read
totoRNAseq Read 1 v2 CCACCGAGATCTACACTCTTTCCCTACACGACG totoRNAseq Read1
N2RevSeq2 CTCGGCATTCCTGCTGAACCGCTCTTCCGATCT totoRNAseq Read2
N2IndSeq AAGAGCGGTTCAGCAGGAATGCCGAG totoRNAseq N2 index Read
STRT-SEQ v3 GATCTAAGCAGTGGTATCAACGCAGAGTACGGG totoRNAseq Read1