Daniel:Notebook/HiResChrPaint/2013-7-9

From ZhangLabWiki
Revision as of 17:35, 9 July 2013 by >Djacobse
Jump to navigation Jump to search

S2 Probe Prep (Started 5/29/2013)

Back to Calendar

ULYSIS Dye Coupling

Based on yesterday's results I think it would be helpful to see what happens if I use all 5 uL of dye available (see ULYSIS protocol)
in one labeling. I'll use the oligo Noi gave me again, as I have a lot and it doesn't waste probe.
1. Add 5 uL DMSO to Alexa dye 488
2. Add 1 uL DNA in 19 uL labeling buffer (Component C)
3. Denature DNA for 5 minutes at 95C; snap cool on ice
4. Add 5 uL dye to sample
5. Incubate for 15 minutes at 80C, snap cool on ice
6. Purify using Centri-Sep Column