Daniel:Notebook/HiResChrPaint/2013-7-12

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Revision as of 18:08, 12 July 2013 by >Djacobse
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S2 Probe Prep (Started 5/29/2013)

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I've been performing some research on the ULS labeling system to see if I can get a good grasp on what's going on before
I try labeling again.  The most telling, I think, is some figures from a poster I got on Invitrogen's webstite.

Research Notes: ULS

A B
File:ULS-LabelingRatio.png File:ULS-ReactionDuration.png
C D
File:ULS-ReactionRatio.png File:ULS-ReactionTemp.png

Summary: A: Optimal labeling ratio for ULS dyes is around 2%, or 1 in 50 bp. This experiment was done on Alexa 488 so it should be directly applicable to my research. B: Increasing reaction time up to 1 hour should only improve labeling. Optimal time is suggested around 15 minutes but I think 20-30 wouldn't be too bad either. C: Reaction ratio