Daniel:Notebook/HiResChrPaint/2013-7-12
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S2 Probe Prep (Started 5/29/2013)
I've been performing some research on the ULS labeling system to see if I can get a good grasp on what's going on before I try labeling again. The most telling, I think, is some figures from a poster I got on Invitrogen's webstite.
Research Notes: ULS
A | B |
File:ULS-LabelingRatio.png | File:ULS-ReactionDuration.png |
C | D |
File:ULS-ReactionRatio.png | File:ULS-ReactionTemp.png |
Summary: A: Optimal labeling ratio for ULS dyes is around 2%, or 1 in 50 bp. This experiment was done on Alexa 488 so it should be directly applicable to my research. B: Increasing reaction time up to 1 hour should only improve labeling. Optimal time is suggested around 15 minutes but I think 20-30 wouldn't be too bad either. C: Reaction ratio