Hosuk:LabNotesMDA/2013-7-26

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Revision as of 19:00, 26 July 2013 by >Hosuki78 (Created page with "*LabNote ===MDA with PGP1F, 11th, Dev v9.1=== *Use new device (v9.1) *Same experiment to [[Hosuk:LabNotesMDA/2013-7-1...")
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MDA with PGP1F, 11th, Dev v9.1

  • Use new device (v9.1)
  • Same experiment to 07/18, but try pushing ALS+Cell into compartments with H2O, not air.


Experiment

  • Start : 5:30pm 07/25, End : 8:30am 07/26 --> about 15hr reaction
  • qPCR run for verification
    • Extract just about 1.1 uL at each tube and run qPCR.



Result

  • Good, considering bad experiment condition
    • First(Left) two chambers had lower amplicons because of less template, but it’s better than pushing with air.
    • Total amount of DNA estimated were lower than previous run (07/18),
    • but the distribution of amount of DNAs was relatively even though no active mixing!
    • It looked enough amount for tagmentation