Daniel:Protocols/ssDNA Column
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ssDNA Column Purification (Zymo)
Zymo: D7010 (20); D7011 (50)
Protocol
1. Add 2 volumes Binding Buffer to the sample; mix well 2. Transfer to IIC Column and centrifuge at 14000 rpm for 1 minute; SAVE THE FLOW THROUGH 3. Add 1 volume 100% EtOH to flow through; mix well 4. Transfer to IC Column and centrifuge at 14000 rpm for 1 minute; discard flow through 5. Add 400 uL Prep Buffer and centrifuge at 14000 rpm for 1 minute; discard flow through 6. Add 700 uL Wash Buffer and centrifuge at 14000 rpm for 1 minute; discard flow through 7. Add 400 uL Wash Buffer and centrifuge at 14000 rpm for 1 minute; discard flow through 8. Centrifuge empty column at 14000 rpm for 2 minutes 9. Transfer to empty 1.5mL centrifuge tube (low bind) 10. Add ≥ 6 uL nfH2O and centrifuge at 14000 rpm for 1 minute