Daniel:Notebook/HiResChrPaint/2013-8-1
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Troubleshooting qPCR (Started 7/31/2013)
PCR 2 (Me)
Since I'm pretty sure that the PCR is working fine, except that the master mix I used wasn't working, I'm going to try again (2 reactions only!) and see if I can't PCR something, one using my MM and one using Noi's.
20 nM OligoPool | 2X Kapa SYBR qPCR MM | 100 uM AP1V41U | 100 uM AP2V4 | 100 uM AP1V61U | 100 uM AP2V6 | 2 mM aa-dUTP | H20 | Total Volume | |
V4S1D | 0.5 | 50 | 0.5 | 0.5 | 0.5 | 0.5 | 0 | 47.5 | 100 |
V4S1N | 0.5 | 50 | 0.5 | 0.5 | 0.5 | 0.5 | 0 | 47.5 | 100 |