Daniel:Notebook/HiResChrPaint/2013-8-1

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Revision as of 21:41, 1 August 2013 by >Djacobse (Created page with "==Troubleshooting qPCR (Started 7/31/2013)== Back to Calendar ===PCR 2 (Me)...")
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Troubleshooting qPCR (Started 7/31/2013)

Back to Calendar

PCR 2 (Me)

Since I'm pretty sure that the PCR is working fine, except that the master mix I used wasn't working, I'm going to try again (2 reactions only!) and see if I can't PCR something, one using my MM and one using Noi's.

  20 nM OligoPool 2X Kapa SYBR qPCR MM 100 uM AP1V41U 100 uM AP2V4 100 uM AP1V61U 100 uM AP2V6 2 mM aa-dUTP H20 Total Volume
V4S1D 0.5 50 0.5 0.5 0.5 0.5 0 47.5 100
V4S1N 0.5 50 0.5 0.5 0.5 0.5 0 47.5 100