Song:Experimental Design For Brainbow

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Aim 1.To generate cell lines random-integrated with brainbow cassette

Aim 2.To introduce inducible Cre into brainbow-integrated cell lines

Aim 3.To establish live cell imaging and automated lineage tracking system

Aim 4.To manually isolate single cell from differentiation culture

Aim 5.To perform single-cell transcriptomic and epigenomic analysis