Blue:RNA-Seq Experiments:09232013
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Fluidigm C1 Run: Human Pyramidal Nuclei
- Samples prepared and sorted by Yun at Scripps (FACS ARIA)
- Bulk sorted nuclei based on PI positive - ~120 nuclei/ul
- Centrifuged nuclei at 2000rpm 4C for 10 min (table-top centrifuge)
- Resuspended in low volume and counted - ~250 nuclei/ul
- Used ERCC diluted 1:200,000 (10 fold less than previous experiment)
- Add 15ul of nuclei to 10ul cell suspension reagent and loaded 20ul onto chip
- Observed chip under microscope and counted occupancy rate: