Blue:RNA-Seq Experiments:09232013

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Revision as of 01:18, 24 September 2013 by >B1lake (Created page with "== Fluidigm C1 Run: Human Pyramidal Nuclei == *Samples prepared and sorted by Yun at Scripps (FACS ARIA) *Bulk sorted nuclei based on PI positive - ~120 nuclei/ul *Centrifuge...")
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Fluidigm C1 Run: Human Pyramidal Nuclei

  • Samples prepared and sorted by Yun at Scripps (FACS ARIA)
  • Bulk sorted nuclei based on PI positive - ~120 nuclei/ul
  • Centrifuged nuclei at 2000rpm 4C for 10 min (table-top centrifuge)
  • Resuspended in low volume and counted - ~250 nuclei/ul
  • Used ERCC diluted 1:200,000 (10 fold less than previous experiment)
  • Add 15ul of nuclei to 10ul cell suspension reagent and loaded 20ul onto chip
  • Observed chip under microscope and counted occupancy rate: