Daniel:Notebook/HiResChrPaint/2013-8-14

From ZhangLabWiki
Revision as of 18:49, 26 September 2013 by >Djacobse (Created page with "=Amino Group Primers= Back to Calendar So the new primers are in. We ordered them a few weeks ago. All the primers have a 5' amino group,...")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

Amino Group Primers

Back to Calendar

So the new primers are in. We ordered them a few weeks ago. All the primers have a 5' amino group, which should be compatible with the Alexa-dye coupling system. Thanks to Matt's results yesterday, I know that when the dye coupling does not work, the Centri-Sep columns yield very little dye. So, before going nuts on making new probes, I'm going to test the water with a simple comparison. For sample 1, I'm going to test the dye using the new primer, plus no aa-dUTP. I'm also going to do a positive control using a standard amplification using the old primers and aa-dUTP. Finally, I'll run a negative control using old primer and no aa-dUTP. I'll do this for both V4S3 and V6S3, since I should test both primer sets.