Hosuk:LabNotes/2013-9-30
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1st Rolonies and ACTB Gene detection in 1st Rolonies together
Goal
- Count ACTB Gene out of 1st rolonies
- Analyze how many ACTBs in all cDNA Rolonies quantitatively
Procedure
- strip previous proves,
- rehybridize Cy5 labeled probe for 1st rolonies, and ATTO 488 labeled probe for ACTB target