Hosuk:LabNotes/2013-9-30

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Revision as of 03:14, 1 October 2013 by >Hosuki78
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1st Rolonies and ACTB Gene detection in 1st Rolonies together

Goal

  • Count ACTB Gene out of 1st rolonies
  • Analyze how many ACTBs in all cDNA Rolonies quantitatively


Procedure

  • strip previous proves,
  • rehybridize Cy5 labeled probe for 1st rolonies, and ATTO 488 labeled probe for ACTB target



PGP1F in 96well plate

  • For testing multiple sets, cells are split in glass bottom 96 well plate.
  • 2 well plates are used, and 8 wells in one plate have cells.

Split condition

  • About 90% confluenced cells in T25 flask
  • After spin dow cells, add 2mL media,
  • transfer 60uL cells in a well, and add 100uL media
  • Let's see how long it takes time for 100% cells in a well.