Rui:10.10.13 discussion

From ZhangLabWiki
Revision as of 22:40, 10 October 2013 by >RuiLiu (Created page with "==10.10.13 discussion== * Current methods Efficiency Ref(10pg) SC(mNPC,mES) SN(nuclei) Smart-seq 10% + + N/A Smart-seq2 40% (est.) ILMN-dT/N6-9 ? + +(?) C1...")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

10.10.13 discussion

  • Current methods
			Efficiency	Ref(10pg)	SC(mNPC,mES)	SN(nuclei)
Smart-seq		10%		+		+		N/A
Smart-seq2		40% (est.)	
ILMN-dT/N6-9		?		+		+(?)

C1/Smart-seq		10-20%				+		+(?)
STRT/C1 (Stenn, UMI)	48%	
Cel-seq		17%
  • Methods plan
# STRT/C1 integrated with frag./PAP
# Cel-seq integrated with frag./PAP
# fine tune PAP: control length
  • Upstream
# cell sorting
# C1 fluidigm
  • Downstream
# data analysis: TSCC, Kun's scripts
# Wei's group