AlanFung:Protocol/MetaMorph Image Analysis

From ZhangLabWiki
Revision as of 23:49, 16 October 2013 by >Alan6017518
Jump to navigation Jump to search

Guide

File:MetaMorph Basic Commands.pdf File:MetaMorph Dropin Commands.pdf

Count Nuclei (P.789 in Dropin Commands)

  • Use this application module to segment images that are used to identify and differentiate cell nuclei.
  • The segmentation labels each isolated and identified nucleus as a different color index to enable you to see a visual separation between nuclei that are close or touching.
  • Source images must be 16-bit images.

Three settings are available to you:

  • Approximate minimum nuclei width, Approximate maximum nuclei width, and the minimum gray level intensity of the nuclei above the local background.
  • Any nuclei-like pattern in the image less than the specified minimum width will be considered to be noise, and will be excluded from the segmentation.

Parameters

Determine Dimensions

Determine Intensity above local background

Example

  • Original Image
  • Segmentation Image
  • Parameters
  • Results